Detecting rare antigen-specific T-cell responses requires assays that are both sensitive and scalable. Traditional methods like flow cytometry often struggle with high-throughput needs or with preserving precious sample material.

In this episode of Listen In, you’ll discover how ELISpot and FluoroSpot assays overcome these challenges. These cell-based immunoassays measure cytokine and antibody secretion at the single-cell level, offering a sensitive, robust, and accessible way to capture rare immune responses.

You’ll learn how ELISpot and FluoroSpot can be applied in vaccine studies, biotherapeutic evaluation, and cell and gene therapy development. The episode also explores practical guidance on assay setup and execution, the advantages of multiplex cytokine detection with FluoroSpot, and how these platforms can be qualified for use in preclinical and clinical trials.

With their unique combination of sensitivity and ease of use, ELISpot and FluoroSpot provide a valuable alternative to flow cytometry, especially when high throughput and efficient data collection are priorities.

Watch the full presentation here: https://events.bitesizebio.com/detecting-rare-t-cell-responses-at

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/